Gloucester Rugby are a professional rugby union club based in the West Country city of Gloucester, England. They play in Premiership Rugby, England's top division of rugby, as well as in the European Rugby Champions Cup.

The club was formed in 1873 and since 1891 has played its home matches at Kingsholm Stadium, on the fringes of the city centre. Their biggest successes are winning the Anglo-Welsh Cup five times: in 1971–72, 1977–78, 1981–82, 2002–03 and 2010–11; and the European Challenge Cup twice: in 2005–06 and 2014–15.

The club has no official nickname but are often referred to as the Cherry and Whites by supporters and the media in reference to the traditional Cherry and white hooped shirts worn by the team. Matches with local rivals Bath and Bristol Bears are referred to as West Country derbies.