The 2010 season for Liquigas–Doimo began in January with the Tour de San Luis and ended in October at the Giro di Lombardia. As a UCI ProTour team, they were automatically invited and obliged to send a squad to every event in the ProTour.

The team won two of the season's three Grand Tours, the Giro d'Italia, with Ivan Basso and the Vuelta a España with Vincenzo Nibali. Franco Pellizotti was expected to ride both of the season's first two Grand Tours, but he was sidelined by the team after the UCI identified him as having irregular blood values as part of their biological passport program, values possibly indicative of doping. Pellizotti fought the charges, proclaiming his innocence, and was later cleared to return to racing. However, delays in his case meant that almost all of his 2010 season was wiped out.